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dr gfp  (Addgene inc)


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    Structured Review

    Addgene inc dr gfp
    Dr Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdrgfp/pDRGFP+(Plasmid+%2326475)/pm41826730-90-12-14
    Average 96 stars, based on 188 article reviews
    dr gfp - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Targeting FZD6 creates therapeutically actionable vulnerabilities for advanced prostate cancer
    Article Snippet: .. The pDRGFP (Addgene, #26475) and pimEJ5GFP (Addgene, #44076) plasmids were transfected into C4-2 cells separately using lipofectamine 3000. ..

    Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness
    Article Snippet: .. For HDR reporter assay, dTAG-NUDT21 HCT116 cells were transfected with pDRGFP (Addgene, #26475) and pCBASceI (Addgene, #26477) together for 6 hours. .. The transfected cells then received different treatments for 18 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells.

    Article Title: Targeting FZD6 creates therapeutically actionable vulnerabilities for advanced prostate cancer.
    Article Snippet: .. The pDRGFP (Addgene, #26475) and pimEJ5GFP (Addgene, #44076) plasmids were transfected into C4-2 cells separately using lipofectamine 3000. ..

    Article Title: Overexpression of the NEK8 kinase inhibits homologous recombination.
    Article Snippet: Live cells were counted and analyzed using Molecular Devices ImageExpress Micro 4 and proprietary MetaXpress software. .. U2OS cells were transfected with a pDRGFP plasmid, mCherry pCAGGs, and either empty vector (pCAGG, negative control) or pCBA (caption on next page) SceI plasmids [38]. pDRGFP (Addgene #26475) and pCBASceI (Addgene #26477) were gifts from Maria Jasin [4,38]. mCherry pCAGGs (Addgene # 41583) was a gift from Phil Sharp [39]. ..

    Article Title: Fadraciclib, a CDK2/CDK9 inhibitor, shows efficacy in biliary tract cancer and synergistic potential with olaparib and JQ1 based on MCL1 expression
    Article Snippet: .. pDRGFP (#26,475) and pCBAScei (#26,477) were purchased from Addgene (Watertown, MA, USA). pDRGFP was transfected by electroporating using Lonza 4D-Nucleofector system and SF Cell Line 4D-NucleofectorTM X Kit L (Lonza, Basel, Switzerland) according to the manufacturer's protocols. pDRGFP-inserted cells were collected through mammalian selection. ..

    Article Title: A novel biguanide-derivative promotes NEDD4-mediated FGFR1 ubiquitination through BMI1 to overcome osimertinib resistance in NSCLC
    Article Snippet: Finally, images were captured using an Olympus FV3000 confocal microscope (Olympus, Tokyo, Japan). .. Cells (3 × 10 5 /well) were transfected with the HR reporter plasmid, pDRGFP (AddGene, Watertown, Massachusetts, USA) in 6-well plates. .. The following day, cells were transfected with the Sce-I-expressing plasmid (pCBASce-I; AddGene,Watertown, Massachusetts, USA).

    Article Title: Fadraciclib, a CDK2/CDK9 inhibitor, shows efficacy in biliary tract cancer and synergistic potential with olaparib and JQ1 based on MCL1 expression.
    Article Snippet: .. pDRGFP (#26,475) and pCBAScei (#26,477) were purchased from Addgene (Watertown, MA, USA). pDRGFP was transfected by electroporating using Lonza 4D-Nucleofector system and SF Cell Line 4D-NucleofectorTM X Kit L (Lonza, Basel, Switzerland) according to the manufacturer's protocols. pDRGFP-inserted cells were collected through mammalian selection. ..

    Reporter Assay:

    Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness
    Article Snippet: .. For HDR reporter assay, dTAG-NUDT21 HCT116 cells were transfected with pDRGFP (Addgene, #26475) and pCBASceI (Addgene, #26477) together for 6 hours. .. The transfected cells then received different treatments for 18 hours before running through a FACSCanto II flow cytometer (BD) to determine the percentage of GFP-positive cells.

    Plasmid Preparation:

    Article Title: APOBEC3C coordinates DDX5 in R-loop resolution and dynamic control of Chk1-mediated stress-responsive circuitry as a prerequisite for gemcitabine resistance in p53-deficient cells
    Article Snippet: .. The pDRGFP (plasmid no. 26475), pimEJ5FP (plasmid no. 44026) and pCBSceI (plasmid no. 26477) were ordered from Addgene. ..

    Article Title: Overexpression of the NEK8 kinase inhibits homologous recombination.
    Article Snippet: Live cells were counted and analyzed using Molecular Devices ImageExpress Micro 4 and proprietary MetaXpress software. .. U2OS cells were transfected with a pDRGFP plasmid, mCherry pCAGGs, and either empty vector (pCAGG, negative control) or pCBA (caption on next page) SceI plasmids [38]. pDRGFP (Addgene #26475) and pCBASceI (Addgene #26477) were gifts from Maria Jasin [4,38]. mCherry pCAGGs (Addgene # 41583) was a gift from Phil Sharp [39]. ..

    Article Title: A novel biguanide-derivative promotes NEDD4-mediated FGFR1 ubiquitination through BMI1 to overcome osimertinib resistance in NSCLC
    Article Snippet: Finally, images were captured using an Olympus FV3000 confocal microscope (Olympus, Tokyo, Japan). .. Cells (3 × 10 5 /well) were transfected with the HR reporter plasmid, pDRGFP (AddGene, Watertown, Massachusetts, USA) in 6-well plates. .. The following day, cells were transfected with the Sce-I-expressing plasmid (pCBASce-I; AddGene,Watertown, Massachusetts, USA).

    Negative Control:

    Article Title: Overexpression of the NEK8 kinase inhibits homologous recombination.
    Article Snippet: Live cells were counted and analyzed using Molecular Devices ImageExpress Micro 4 and proprietary MetaXpress software. .. U2OS cells were transfected with a pDRGFP plasmid, mCherry pCAGGs, and either empty vector (pCAGG, negative control) or pCBA (caption on next page) SceI plasmids [38]. pDRGFP (Addgene #26475) and pCBASceI (Addgene #26477) were gifts from Maria Jasin [4,38]. mCherry pCAGGs (Addgene # 41583) was a gift from Phil Sharp [39]. ..

    Selection:

    Article Title: Fadraciclib, a CDK2/CDK9 inhibitor, shows efficacy in biliary tract cancer and synergistic potential with olaparib and JQ1 based on MCL1 expression
    Article Snippet: .. pDRGFP (#26,475) and pCBAScei (#26,477) were purchased from Addgene (Watertown, MA, USA). pDRGFP was transfected by electroporating using Lonza 4D-Nucleofector system and SF Cell Line 4D-NucleofectorTM X Kit L (Lonza, Basel, Switzerland) according to the manufacturer's protocols. pDRGFP-inserted cells were collected through mammalian selection. ..

    Article Title: Fadraciclib, a CDK2/CDK9 inhibitor, shows efficacy in biliary tract cancer and synergistic potential with olaparib and JQ1 based on MCL1 expression.
    Article Snippet: .. pDRGFP (#26,475) and pCBAScei (#26,477) were purchased from Addgene (Watertown, MA, USA). pDRGFP was transfected by electroporating using Lonza 4D-Nucleofector system and SF Cell Line 4D-NucleofectorTM X Kit L (Lonza, Basel, Switzerland) according to the manufacturer's protocols. pDRGFP-inserted cells were collected through mammalian selection. ..



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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    pdrgfp reporter plasmid  (Addgene inc)
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
    Pdrgfp Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    pdrgfp reporter plasmid - by Bioz Stars, 2026-09
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    Image Search Results


    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control

    doi: 10.64898/2026.02.17.706442

    Figure Lengend Snippet: a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

    Article Snippet: Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions.

    Techniques: Western Blot, Single Cell Gel Electrophoresis, Two Tailed Test, Reporter Assay, Transfection, Control, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein Kinase Assay, Protein Extraction, Activity Assay, Kinase Assay, Transformation Assay, Fluorescence, Phospho-proteomics